BACKGROUND: Multi-drug Resistant (MDR) bacteria could lead
to treatment failure of infectious diseases and could be transferred
by non-potable water. Few studies have investigated occurrence of
Antibiotic Resistance Genes (ARGs) among bacteria including
Aminoglycoside Modifying Genes (AMGs) from Drinking Water
Distribution Systems (DWDS) in Nigeria. Here, we aimed at
characterization of AMGs from DWDS from selected states in
southwestern Nigeria.
METHODS: One hundred and eighty one (181) MDR bacteria that
had been previously characterized using 16S rDNA and showed
resistance to at least one aminoglycoside antibiotic were selected
from treated and untreated six water distribution systems in
southwestern Nigeria. MDR bacteria were PCR genotyped for three
AMGs:aph (3 ́ ́) c , ant (3 ́ ́) b and aph(6)-1d d .
RESULTS: Out of 181 MDR bacteria genotyped, 69(38.12%) tested
positive for at least one of the genotyped AMGs. Highest (50,
27.62%) detected gene was ant (3”) c followed by aph (3") c (33,
18.23%). Combination of aph(3") c and ant (3") b in a single bacteria
was observed as the highest (14, 7.73%) among the detected gene
combination. Alcaligenes sp showed the highest (10/20) occurrence
of ant (3") b while aph(3") c was the highest detected among Proteus
sp (11/22). Other bacteria that showed the presence of AMGs
include: Acinetobacter, Aeromonas, Bordetella, Brevundimonas,
Chromobacterium, Klebsiella, Leucobacter, Morganella, Pantoae,
Proteus, Providencia, Psychrobacter and Serratia.

Published: 2019-05-28