In vitro Nematicidal (Anthelmintic) Property of the Seed Extracts of Anamirta cocculus (Linn.) Against Pheretima posthuma (L. Vaill.)

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Paul V.I.
Umer Qadir

Abstract

Anamirta cocculus is a wild climber having antibacterial and piscicidal properties. Itis used in Ayurvedic and folk medicines. The primary objective of the present studywas to evaluate the in vitro nematicidal or nematodicidal (anthelmintic) activity ofmethanol, chloroform and petroleum ether extracts of the seeds of A. cocculus using theearthworm Pheretima posthuma as an experimental model. After calculating the 1⁄2 an hourLC 50s of all the extracts, the time taken for paralysis as well as death of the worms in eachof the extracts was tested using three concentrations each viz.,10, 15 and 20 mg mL –1 . Allthe three extracts produced significant anthelmintic activities in a dose dependentmanner. The nematicidal activities of the extracts were either significantly higher ormore or less equal to the reference drug Albendazole. Highest nematicidal activity wasshown by the methanol extract (20 mg mL –1 ) with the paralysis and death time of 30.3and 60.2 minutes respectively. Moreover, methanol extract exhibited an increased paralyticas well as nematicidal effect over the reference drug Albendazole at all the givenexperimental concentrations. The results indicated the strong probability of the A.cocculus seeds as the potential raw material for developing an effective nematicide thatmay be highly useful in controlling the plant parasitic nematodes.

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How to Cite
V.I., P., & Qadir, U. (1). In vitro Nematicidal (Anthelmintic) Property of the Seed Extracts of Anamirta cocculus (Linn.) Against Pheretima posthuma (L. Vaill.). Ethiopian Journal of Applied Science and Technology, 4(1), 65-75. Retrieved from https://journals.ju.edu.et/index.php/ejast/article/view/857
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Articles
Author Biographies

Paul V.I., Tamil Nadu, INDI

Corresponding author: Dr. V.I. Paul, Associate Professor, Department of Zoology ,
Annamalai University, Annamalainagar 608 002, Tamil Nadu, INDIA,
Tel: +91 9443046081, E-mail: drissacpaul@gmail.com

Umer Qadir, Tamil Nadu,India

Department of Zoology, Annamalai University, Annamalainagar 608 002, Tamil Nadu,
India